Rehydration of an IPG strip for 2D electrophoresis. 12 hours is recommended for rehydration of an IPG strip. What could be the problem if I increased the. IPG gel strips rehydration. Hydration was performed overnight in the Pharmacia reswelling cassette with 25 ml of a solution containing urea (8 M), CHAPS (2%.
This protocol describes a method for rehydration of IPG gel strips in preparation for their use for isoelectric focusing (IEF) on immobilized pH gradient (IPG) gels. This can cause proteins within the sample to become less soluble, For cup- loading, IPG dry strips are first rehydrated with rehydration buffer.
In this study, Saccharomyces cerevisiae yeast lysate is used to demonstrate how a simple wash procedure can improve IEF of IPG strips passively rehydrated in. IPG strips have to be rehydrated to their original thickness of mm. IPG strip rehydration should be performed for at least 12 h or overnight at.